Mushroom Grow Kit 4LBS – Inject-And-Forget Bag
£39.99
1800 grams of substrate! To the 1000 gram harvest and beyond!
Finally. A mushroom Growing Kit that will work every time.
This mushroom growing kit is super easy to use. The special Mushroom substrate is held in a extra large all in one mushroom growing bag. The bag allows your spores to germinate in the substrate and the resulting mycelium to fruit all inside the same bag. Gas exchange is taken care of by an extra large filter patch and the self healing injection port allows the spores to be injected into the substrate with ease. It is a continuation of our world famous Inject-and-Forget mushroom growing kits. An automatic Mushroom Growing kit from us to you.
Please Note: Spores are not included.
Due to age verification, confirmation of payment and nature of product, there can be a 3 – 7 day delay prior to shipping on this item.
Description
Mushroom Grow Kit 4LBS – Inject-And-Forget Bag with Oxygen Release Agents
Version 3.0 – 1800 grams of substrate! To the 1000 gram harvest and beyond!
Pictured next to other popular products for size comparison.
NOT sold with spores.
——————————–
Finally. A mushroom Growing Kit that will work every time.
The Inject-and-Forget Bag system, invented in the UK by MushBuddies. This is a WORLD FIRST!
They said it was NOT possible! They were wrong!
This mushroom growing kit is super easy to use. The special Mushroom substrate is held in a extra large all in one mushroom growing bag. The bag allows your spores to germinate in the substrate and the resulting mycelium to fruit all inside the same bag. Gas exchange is taken care of by an extra large filter patch and the self healing injection port allows the spores to be injected into the substrate with ease. It is a continuation of our world famous Inject-and-Forget mushroom growing kits. An automatic Mushroom Growing kit from us to you.
Have you been having trouble growing your mushrooms? Do you find all the fanning and misting a hassle, or have you been looking at the equipment costs and thinking “I wish I could afford that”? Well you’re not alone!
When we first started cultivating mushrooms, we grew more mold than anything else.
We always wished we could just get a big bag of substrate, inject the bag with spore solution and then almost forget about it until it was fruiting with mushrooms. No fanning, no misting, no equipment, no fuss! Just bags FULL of mushrooms!
WELL NOW YOU CAN and WITH ALMOST NO CHANCE OF CONTAMINATION.
For the third time in our history, MushBuddies have managed to design a mushroom growing kit to do just that.
WHAT YOU WILL NEED:
You will need a 20ml syringe for the 4lbs bag.
You will need a heat mat with thermostat or heated propagator, like our very own GrowBuddy60 which is able to fit 2 x 4Lb Inject-and-Forget Bags.
You will need a dim light source around 4500k-6500k. Standard room lighting and indirect sunlight also works perfectly.
You will need a spore syringe or syringe of gourmet liquid culture.
You will need a Easy Glide Bag Sealer as the bags are capable of many flushes and the bag sealer with do just as its name says between flushes/harvests.
Things not to do!
1] The needle is sterile from the packet. Do not wipe it or heat it. Just connect and go.
2] If the syringe seems blocked, do not remove it from the injection port. Just pull the needle back a tiny bit and press harder on the plunger.
3] Do not open the bag until you want to harvest. The bag is a sealed unit and keeps contamination out and moisture in.
HOW TO USE THIS MUSHROOM GROWING KIT:
1] Connecting your syringe to your needle.
Open the end of the needle packet and then unscrew the black syringe lock tip from the syringe and screw the needle in its place. Leave the protective needle cap on the needle until you are ready to use.
2] Injecting your spores into the bag.
Do not wipe or heat sterilise the needle for this mushroom growing kit only wipe the black self healing injection port on the mushroom growing bag.
Wipe down your injection port with the included alcohol swab.
Remove the protective needle cap and push the needle into the black injection port on the bag. Push on the plunger to inject the solution into the bag. Remove needle and replace needle cap.
3] Massage the substrate
Take time to massage the substrate and break up any clumps, spreading the spore solution evenly throughout the bag. Do not open the bag. But do spend 5 minutes on this part.
Why?
If you can massage the bag so as to have at least on spore per square centimeter of substrate you will have a fully colonised bag after 14 days. If you don’t evenly distribute the spores you will have a section with no life and this will delay your project and need a secondary shake up to aid colonisation.
When the substrate is all mixed up, lay the bag on its side and shake the bag to evenly level the substrate. The bag should now look like an elongated tent with the substrate evenly distributed in the bottom just like in the pictures above. Do not compress the substrate. The substrate should be all broken up and airy.
Now mushrooms love to side pin and most pins will appear on the side walls of the colonised substrate. For this reason, making the substrate into a ‘loaf’ shape with taller sides and a thinner top will promote the heaviest harvest.
How to colonise the substrate.
First tape your silver temperature probe of the thermostat to the heat mat. You do not want it to fall off. Now place the bag or one of your bags on top of the temperature probe on the heat mat or propagator and check temperature is set to 26-27C via the thermostat. Heat mats and Growbuddies purchased from our store will be Pre-set at 26-27c. Our systems are always plug and play meaning plug the heat mat in and it will maintain the perfect conditions for you day and night, so now you can sit back and relax.
The Mushbuddies GrowBuddy60 and Heat Mats come PRESET for you so you don’t have to worry!
To help the bags work, you can house your heat mat and Inject-and-Forget bag in a small storage box if you want; simply drill 4x 8mm holes on each side of the box for air flow, make 2 holes down low on each side close to the base of the tub and two holes on each side up high close to the lid of the tub.
This makes you a little incubator, helping to maintain a nice warm environment around your bag. The holes you have drilled will allow the box to breath, bringing fresh air flow past your bags air filter.
Do not enclose the Inject-and-Forget bag in a sealed box, the bag must be able to expel moisture and gases via the Air filter. Evaporation inside the bag will depend on the filter patch being able to breath; the reason why the propagators with lids work so well is they have a nice flow of warm air (and therefore slightly drier air) to help the bags breath.
- Keep the bag in the dark until it is fully colonised and ready to fruit.
Chill and wait for life to appear, do not handle the bags as the mycelium is trying to do its job and is very delicate at all stages.
Very soon the mixed-up substrate will be fully colonised and you’re ready to start the fruiting process.
If you did not mix the spores into all the substrate well enough, you may have some barren areas. Most of the time these areas will be colonised by the mycelium. It is common for a small patch of substrate to remain uncolonised by the filter patch. This shows that the evaporation and breathing process of the Inject-and-Forget system is working well. If you have barren areas at 3-4 weeks after injection. You can break all the substrate up again and remix. Full colonisation should be complete 5 days after.
If massage, temperatures and conditions are correct, full colonisation of the substrate can be as quick as two weeks meaning the bag has turned all white and is ready to fruit. If the bags are kept too warm, too cold or can’t breathe properly, full colonisation of the bag may take up to 5-6 weeks or longer, so maintaining the correct environment is IMPORTANT!
If you do drop the bag or need to break up the substrate at this time you may delay the fruiting process…. Keep calm and carry on. There is nothing you can do, but life will still prevail.
How to fruit
Now you are ready to fruit! To start the fruiting process, turn on your lights, 12/24 works great and keep maintaining the temperature at 26-27C.
Make sure the light source is not to bright for the life inside the Mushroom Growing bag. Low light works great bright light does not. Plants light bright light and mushroom light dim light.
After 10-20 days in the correct environment you will see the pins forming along the sides of the cake. These tiny dots are the mushroom pins that will grow into nice big juicy mushrooms.
Sit back and just watch them grow!
Different strains will take different times, so please be patient. The life form that you are caring for will be able to do its job with the correct conditions and the correct amount of time. Try not to handle the bags and do place the bags in a heated propagator or on a heat mat in a nice warm room. Being warm and cosy is exactly what the mycelium wants.
The mushrooms will turn from the small pins into identifiable mushrooms. If you have one or two mushrooms that have started early, wait until those mushrooms are mature then cut the end of the bag open to remove them. Close the bag back up using a Easy-Glide Bag Sealer and allow the other mushrooms to mature.
If the bags have been kept too cool, the mushrooms will be short adapting to the lower temps. If the bags have been kept with both base and ambient temperature at 26-27 C, the mushrooms can grow huge with 10inch long stems!
When the majority of the mushrooms are mature (or the bag is full) please cut open one end of the bag. Peel the mushroom clusters away from the substrate including any under developed mushrooms.
Harvest before any spores (black soot) fall out of the mushroom caps. If this happens, Keep calm and carry on.
Wash your hands and always keep the Mushroom Substrate loaf inside the bag at all times to reduce chances of contamination.
How to get more flushes
If you want to get another flush after harvesting the mushrooms. Turn on the cold tap in the kitchen and add enough water to the bag so the mushroom substrate loaf sits in 1 inch of water. The trace amount of chlorine in the tap water helps cleanse the loaf while it soaks. Seal the bag up and leave for 12 hours, then drain for 30 min sat at an angle. Close the grow bag with an Easy-Glide Bag Sealer and place back in fruiting conditions. It will take a similar amount of time for subsequent flushes to show.
OMBucha’s brown rice flour substrate mix has oxygen release agents that will slowly release oxygen for your mushrooms during growth. The agents work by time release; meaning they will not hamper germination or colonisation… only releasing when needed. The oxygen release rate will increase during fruiting and so will actively push out any CO2 generated as the mushrooms grow.
Temperature and timing are important with this system, that’s why we strongly recommend the use of a temperature controlled heat mat. Colder temps will lead to reduced yield and longer waiting times!
With temperatures of between 26-27C, you can expect a completely NO FUSS FIRST FLUSH YIELD OF BETWEEN 500-1000g of fresh mushrooms. The average first flush yield during product testing was a healthy 723g.
NOTE:
We sell spores for microscopy.
Please be aware that the cultivation of Psilocybe cubensis is prohibited in the UK.
Disclaimer
Please be aware that the germination of spores from the Psilocybe genus is illegal in many countries, including the UK. Always do your thorough research before purchasing. By purchasing, you are indicating that you are aware of your local laws. You also waive any liability towards FitnessBodyStore ltd t/a OMBucha if you act outside your laws.
All of our spores are sourced from trusted vendors in Austria, where the cultivation of magic mushrooms is legal.
Images provided are for informational and educational reference only.
Images provided for informational and educational reference only and originate from cultivators and labs outside the UK. Cultivation of this species is illegal in many countries including the United Kingdom. Please check your local regulations.
Additional information
Weight | 2.5 kg |
---|
Learn more about our partners at Atlas Biomed and our partnership team here.
Belonging to a haplogroup provides information about where your ancestors lived, how they moved around the planet and where your closest relatives are now.
Types of risk factors: – external (e.g., environment, economic situation, profession) – characteristics of the human body (e.g., elevated blood cholesterol, arterial hypertension, hereditary predisposition) – lifestyle (smoking, diet, exercise)
Disease risk is the sum of all risk factors, which is why Atlas users are invited to fill in the Health and Lifestyle Survey to get the most accurate assessment of their individual risk.
Such diseases are the result of interaction between multiple genetic and external factors that lead to a family predisposition to the disease, without a clear Mendelian inheritance pattern (i.e., classical genetic).
For example, if two DNA sequences - AAGCCTA and AAGCTTA - differ by a single nucleotide, then there are two alleles: C and T. Such point mutations are considered single nucleotide polymorphisms (SNPs).
DNA contains information on the structure of various types of RNA and proteins. It is made up of four nucleotides, known as adenine, guanine, thymine and cytosine or A, G, T, C for short. They are connected together in a specific pattern: adenine binds only to thymine and guanine only to cytosine. These nucleotides encode information about every protein in the body, thus determining the phenotype of a person (i.e., the set of observable characteristics).
It is a rolling subscription, so you will receive a test kit every 3 months. You can choose to terminate it whenever you like. To cancel the subscription, just contact us via email hello@ombucha.com.
If your first order contains both the OMBucha® DNA and Microbiome Tests, the delivery is free of charge.
There exists more than 20,000 scientific articles that have been published relating to the gut microbiome and its relationship with various health conditions over the past decade.
Every single month, world over, new research is released in this field. We used these publications to develop an interpretation system that is now featured in our microbiome test.
This research has firmly established that bacteria in the microbiome are not simply ‘good’ or ‘bad’ species. Rather, how microbes participate in health or illness is dependent on their abundance in the overall community and how they relate to one another. The recommendations provided on your personal account have been developed on the basis of microbiome research by doctors and scientists.
16S ribosomal RNA (or 16S rRNA) is the component of the 30S small subunit of a prokaryotic ribosome that binds to the Shine-Dalgarno sequence. The genes coding for it are referred to as 16S rRNA gene and are used in reconstructing phylogenies, due to the slow rates of evolution of this region of the gene. Carl Woese and George E. Fox were two of the people who pioneered the use of 16S rRNA in phylogenetics in 1977.
Multiple sequences of the 16S rRNA gene can exist within a single bacterium.
Incorporating testing via the OMBucha® gut microbiome test kit is probably your most cost effective, accessible and accurate method to truly analyse & track your gut competition & health.
We test your DNA and microbiome, using the latest technology from Illumina, a world leader in the field of molecular analysis. Your genetic data is analysed in a certified EU laboratory in the Netherlands, accredited with ISO 15189.
Learn more about our home gut microbiome testing.
Why should I take a microbiome test?
The OMBucha® home Microbiome Test analyses the types of bacteria present and their proportion in the overall microbiome. It provides information about the various functions of your gut bacteria, like the extent to which they protect you against certain diseases and inflammation, as well as what vitamins they synthesise. You will also get recommendations to improve and maintain the balance of your microbiome by adding specific foods to your diet.
Benefits of Gut Microbiome Testing:
- Learn how microbes protect you from disease
- Understand how diet affects gut bacteria
- Optimise microbe’s vitamin synthesis
- Dietary fibre breakdown and butyrate synthesis
- Personalised food recommendations
- Probiotics and beneficial bacteria report
What You Will Learn
- Health Your microbiome health score and protection from 5 disease risks
- Nutrition Proportion of probiotics and beneficial bacteria, micronutrient synthesis potential and diversity score
- Foods Weekly personalised food recommendations to improve your microbiome health in 17 areas
- Bacterial composition List of bacteria found in your microbiome (%) and what enterotype you belong to
Learn more about home microbiome testing here.
SUBSCRIBE & SAVE (5% OFF STANDARD PRICES)
Get 5% off your subscriptions for as long as you stay subscribed.WHAT IS SUBSCRIBE & SAVE
- A completely flexible subscription to suit you. No contract – cancel at any time.
- You can now buy a subscription of OMBucha® Kombucha and save both time and money.
- You choose how often you want a delivery, from weekly, bi-monthly or monthly.
- Take the hassle out of reordering and let us do the hard work – everything is set on autopilot!
- Full support: we are always at the end of the email/phone/live chat.
*Kombucha will continue to mature and will eventually become Kombucha vinegar which has a multitude of uses of its own: use it in salad dressings, skin moisturiser, as a hair tonic, or as a marinade.
The majority of kombucha sold on the market, like OMBucha® is raw, and therefore biologically active. The fermentation process continues as long as bacteria and yeast have sugars to feed on. Yeast is temperature sensitive, and cold temperatures keep them less active.
Trace amounts of ethanol are naturally produced by the fermentation process. Keeping kombucha cold is an important means to ensure the quality remains consistent and compliant.
If exposed to warm or hot temperatures, the fermentation continues rapidly and the carbon dioxide will build up quickly. The results could be anywhere from an excess carbonation upon opening to an exploding or broken bottle. It is important to keep commercial kombucha refrigerated at all times to prevent any mishaps. Good thing it is so delicious, leaving it in the bottle almost never happens!
- cholesterol
- diabetes
- healthy liver function
- removing toxins
- destroying free radicals
- chemo-preventative
- anti-microbial/anti-fungal properties
Health Benefits of drinking Kombucha
Read the full article here on why craft OMBucha® is great for your gut and health. In the United Kingdom, we're not allowed to make any claims about Probiotics - as can be seen here: Article 13.1 2009;7(9):1247 from Great Britain nutrition and health claims (NHC) register - GOV.UK (www.gov.uk). So you won't find any information or claims related to Probiotics in relation to any of our products in the UK. Please do not ask us probiotic or gut health related questions, rather, please do your own research from Google and come to your own conclusions and of course, always discuss health matters with a fully qualified and highly trusted (hopefully pillar of health) GP at all times! May Improve Digestion As suggested by many enthusiasts, empirical data proposes that Kombucha tea potentially promotes healthy digestion. Many Kombucha lovers are attributing to the process of fermentation with a live colony of bacteria and yeast, that the beneficial application of fermented tea, may help sustain the gut health and provides relief from various abdominal disorders including chronic constipation and diarrhoea symptoms. The digestive enzymes present in it enhance the effects of glucuronic acid and aid in the breakdown of proteins and saccharides thereby, potentially assisting normal regulation of the digestive system. Many enthusiasts believe Kombucha possesses antibiotic properties i.e. bactericidal and bacteriostatic which works against a range of pathogens and is used for therapeutic purposes in human as well as veterinary treatments - though this has not been factually assessed in the UK, and variation in ones Kombucha brewing is derived from the source of the SCOBY. Could Detoxify the Body Kombucha may have a remarkable reputation for the ability to detoxify the body according to many online sources. Some proponents of Kombucha attribute this potential to a powerful detoxifier glucuronic acid that may be present in some Kombucha brews, which theoretically binds some toxins from the liver and then assisting eliminating them out of the body via kidneys, from what we have researched. Possible Antioxidant Agent Kombucha contains profuse amounts of organic acids like glucuronic acid, of which, some have been noted globally, though not in the UK, to possibly work as powerful antioxidants which may help in shielding the body from oxidative damage. The possibility of antioxidants present in some Kombucha teas, could look out for the oxygen free radicals and neutralize their effects thereby, some enthusiasts speculate that this might be helping repairing and protecting the body from diseases and inflammations. Maybe Even Liver Support Another suggested action purported by kombucha lovers, is its possible hepatic-protective effects. A research study conducted on kombucha tea has shown that its tea helps in restoring the levels of glutathione and some specialists outside of the UK have speculated that this may represent a remarkably effectual way in reducing possible hepatotoxicity. A comparative study conducted on black tea, kombucha tea, and enzyme-processed black tea has indicated the efficacy and superiority of kombucha tea in possibly providing hepatic protection against toxicity maybe attributing to its antioxidant potential, as compared to the latter. Possible Anti-microbial Effects Kombucha tea has even been suggested by some to possess anti-microbial properties which may prove effective against a variety of pathogens. An investigative research has revealed that apart from acetic acid, it contains other promising anti-microbial components which even at neutral values of pH, may possibly provide protection against a range of microorganisms including Staphylococcus aureaus, Staphylococcus epidermis, Escherichia Coli, Salmonella enteriditis, Pseudomonas aeruginosa, Listeria monocytogens, and Helicobacter pylori. The specific bacteria and yeast strains in the kombucha are what make it act the way it does, and what produces the fizz and flavour of kombucha. Not all kombucha cultures will contain the exact same strains, but these are some that have been recorded in studies: Acetobacter [2] is an aerobic (requiring oxygen) bacteria strain that produces acetic acid and gluconic acid. It is always found in kombucha. Acetobacter strains also build the SCOBY mushroom. Acetobacter xylinoides and acetobacter ketogenum are two strains that you might find in kombucha. Saccharomyces [2] includes a number of yeast strains that produce alcohol and are the most common types of yeast found in kombucha. They can be aerobic or anaerobic (requires an oxygen-free environment). They include Saccharomycodes ludwigii, Saccharomycodes apiculatus, Schizosaccharomyces pombe, Zygosaccharomyes, and Saccharomyces cerevisiae. Brettanomyces [2] is another type of yeast strain, either aerobic or anaerobic, that are commonly found in kombucha and produce alcohol or acetic acid. Lactobacillus [2]: A type of aerobic bacteria that is sometimes, but not always, found in kombucha. It produces lactic acid and slime. Pediococcus [2]: These anaerobic bacteria produce lactic acid and slime. They are sometimes, but not always, found in kombucha. Gluconacetobacter kombuchae [2] is an anaerobic bacteria that is unique to kombucha. It feeds on nitrogen that is found in tea and produces acetic acid and gluconic acid, as well as building the SCOBY. Zygosaccharomyces kombuchaensis [3] is a yeast strain that is unique to kombucha. It produces alcohol and carbonation as well as contributing to the mushroom body. Glucuronic acid Maybe the body's most important detoxifier. When toxins enter the liver this acid could bind them to it and assist in flushing them out through the kidneys. It has been suggested that once bound by glucuronic acid toxins cannot escape. A product of the oxidation process of glucose, glucuronic acid is one of the more significant constituents of Kombucha. As a promising potential detoxifying agent it might be one of the few agents that can cope with pollution from the products of the petroleum industry, including all the plastics, herbicides, pesticides and resins. It could help kidnap the phenols in the liver, which are then eliminated easily by the kidneys. Reportedly, Kombucha could be very helpful for allergy sufferers. Another by-product of glucuronic acid are the glucosamines, the structures associated with cartilage, collagen and the fluids which lubricate the joints. It is this function that has been suggested might allow Kombucha to be effective against arthritis. Glucuronolactone, glucuronic acid is involved in the metabolism of detoxification in the liver whose glucuronyl rest of the body is linked to a group of foreign compounds or any of the metabolites in the body so that they become soluble in water and are excreted in the urine. Glucuronic acid should be distinguished from gluconic acid because it is linear and the gluconic acid is formed by oxidation of the different carbon glucose atom. Lactic Acid Essential for the digestive system. Assist blood circulation, helps prevent bowel decay and constipation. Aids in balancing acids and alkaline in the body and believed to help in the prevention of cancer by helping to regulate blood pH levels. Acetic Acid A powerful preservative and it inhibits harmful bacteria. Usnic Acid A natural antibiotic that can be effective against many viruses. Oxalic Acid An effective preservative and encourages the intercellular production of energy. Malic Acid Helps detoxify the liver. Gluconic Acid Produced by the bacteria, it can break down to caprylic acid is of great benefit to sufferers of candidiasis and other yeast infections such as thrush. Butyric acid Produced by the yeast, protects human cellular membranes and combined with Gluconic acid strengthens the walls of the gut to combat yeast infections like candida. Kombucha also contains a variety of other nutrients, particularly various acids and esters that give the drink its characteristic tang and fizz. Included in these components is gluconic acid, which is the primary difference between the makeup of kombucha and the makeup of apple cider vinegar. Flavonoids Flavonoids are part of a large class of chemicals called polyphenols that occur naturally in plants. Flavonoids, which are found in a variety of fruits and vegetables as well as in tea and red wine, are thought to maintain health in part by combating oxidation. That's why they're called antioxidants. Antioxidants help the body's cells resist damage by free radicals. Tea has one of the highest total flavonoid contents of all plants at 15% of the leaf by dry weight and is the major source of flavonoids in the UK diet, providing approximately 80% of dietary flavonoids for the population as a whole. So don't stop drinking your tea! The actual bacteria, sugar, and acid content of kombucha depend on many factors, including the initial culture, the type of tea used, the type of sugar used, the strength of the tea, the type of water, the brewing time, the culturing temperature, and more. Due to the nature of kombucha, it is not possible to state an exact microbial composition for Kombucha. [1] While different SCOBYs may vary in their exact makeup, what is common to all kombuchas is gluconic acid, acetic acid, and fructose. [2]SOURCES1. Ai Leng Teoha,, Gillian Heard, Julian Cox.(2044). Yeast ecology of Kombucha fermentation. International Journal of Food Microbiology, 95(2), 119-126. doi:10.1016/j.ijfoodmicro.2003.12.020 2. Jayabalan, R., Malini, K., Sathishkumar, M., Swaminathan, K., & Yun, S. E. (2010). Biochemical characteristics of tea fungus produced during kombucha fermentation. Food Science and Biotechnology, 19(3), 843-847.
3. Kurtzman, C. P., Robnett, C. J. and Basehoar-Powers, E. (2001), Zygosaccharomyces kombuchaensis, a new ascosporogenous yeast from ‘Kombucha tea’. FEMS Yeast Research, 1: 133–138. doi: 10.1111/j.1567-1364.2001.tb00024.x
What strains of bacteria & yeasts are present in Kombucha?
In the United Kingdom, we're not allowed to make any claims about Probiotics - as can be seen here: Article 13.1 2009;7(9):1247 from Great Britain nutrition and health claims (NHC) register - GOV.UK (www.gov.uk). So you won't find any information or claims related to Probiotics in relation to any of our products in the UK. Please do not ask us probiotic or gut health related questions, rather, please do your own research from Google and come to your own conclusions and of course, always discuss health matters with a fully qualified and highly trusted (hopefully pillar of health) GP at all times! The yeasts and bacteria commonly identified as Acetobacter xylinum (bacteria) and Saccharomyces (yeast) have been considered the key players in Kombucha Mushroom Tea ferments. However, as research into kombucha continues and as recently as Feb 2007 specific strains of both bacteria and yeasts have been identified. Similarly to milk‐derived kefir, the exact microbial composition of kombucha cannot be given because it varies. It depends on the source of the inoculum for the tea fermentation. All kombucha cultures should contain the bacteria species: Acetobacter or Gluconobacter, these bacteria are responsible for producing Gluconic and Acetic acid which make Kombucha uniquely kombucha. A typical Kombucha ferment may included the following strains (not always):Bacteria Strains:
- Bacterium gluconicum
- Bacterium xylinum
- Acetobacter xylinum
- Acetobacter xylinoides
- Acetobacter Ketogenum
Yeast Strains:
- Saccharomycodes ludwigii
- Saccharomycodes apiculatus
- Schizosaccharomyces pombe
- Zygosaccharomyes
- Saccharomyces cerevisiae
Acids and others:
- Acetic acid
- Acetoacetic acid
- Benzoic acid
- propenyl ester
- Benzonitrile
- Butanoic acid
- Caffeine
- Citric acid
- Cyanocobalamin
- Decanoic acid
- Ethyl Acetate
- Fructose
- d-Gluconic acid
- Glucose
- Hexanoic acid
- Itaconic acid
- 2-Keto-gluconic acid
- 5-Keto-gluconic acid
- 2-Keto-3-deoxy-gluconic Lactic acid
- Niacinamide
- Nicotinic acid
- Pantothenic acid
- Phenethyl Alcohol
- Phenol, 4-ethyl
- 6-Phospho gluconate
- Propionic acid
- Octanoic acid
- Oxalic acid
- Riboflavin
- d-Saccharic acid
- (Glucaric acid)
- Succinic acid
- Thiamin - plus 40 other acid esters in trace amount.